Resistance in faba bean viciafaba to bean yellow mosaic for 20 accessions, which were replicated virus. The crop is affected by a number of virus diseases, the most important are those persistently transmitted by aphids such as faba bean necrotic yellows virus fbnyv and. A single rep protein initiates replication of multiple. Symptoms are variable and range from no symptoms to systemic necrosis to plant death bean yellow mosaic virus bymv. Faba bean necrotic yellows virus fbnyv belongs to the nanoviruses, plant viruses whose genome consists of multiple circular singlestranded dna components. Two divergent isolates of turnip yellows virus from pea and. Eleven distinct dnas, 5 of which encode different replication initiator rep proteins, have been identified in two fbnyv isolates. Host range of faba bean necrotic yellows virus and potential. Viruses that are clearly members of the currently established geminivirus genera, begomovirus, topocuvirus, curtovirus and mastrevirus are indicated in green, orange, blue and pink respectively. Broad bean wilt virus 1 bbwv1 and broad bean wilt virus 2 bbwv2 are members of the genus fabavirus, with genomes of two positivesense singlestranded rnas separately encapsidated in 2530nm diameter nonenveloped icosahedral particles. These and 2 necrotic isolates readily infected 5 genotypes genotypes subject category. Faba bean necrotic yellows virus how is faba bean necrotic yellows virus abbreviated. To obtain a better understanding of this nanovirus variability in morocco. Nov 17, 2017 faba bean necrotic yellows virus fbnyv genus nanovirus.
Summarises the symptoms, infection cycle, disease risk and control recommendations for rust disease uromyces viciaefabae in faba bean. Organism names see more details faba bean all caused non necrotic reactions in danja and 90l42307. Mosaic, dark green islands, vein banding, figure 10. Diversity and occurrence of chickpea chlorotic dwarf virus on. The genome of a novel virus of the house cricket consists of a 2,517nucleotide nt circular singlestranded dna ssdna molecule with 4 open reading frames orfs.
Sep 20, 2019 the low incidence of cpcdv infection in chickpea and lentil plants with symptoms might be explained by infection with other viruses, such as blrv, pea enation mosaic virus. Resistance of faba bean accessions to bean yellow mosaic. The most important viruses which infect this crop in the mediterranean region are bean leafroll virus blrv, bean yellow mosaic virus bymv, pea seedborne mosaic virus psbmv, and pea enation mosaic virus pemv1 and 2. Faba bean is cultivated under rainfed and irrigated conditions and is distributed in more than 55 countries. The main source of this virus is lucerne, but it has a wide host range which includes the temperate pulses chickpeas, faba beans, field peas and lentils as well as pasture legumes and perennial weeds. Faba bean necrotic yellows virus isolate sv29288 fbnyv. Resistance in faba bean viciafaba to bean yellow mosaic virus. They have icosahedral virions, about 25 nm in diameter, and bipartite, singlestranded positivesense rna genomes lisa and boccardo, 1996. The species name of fbnyv is derived from the symptoms induced on faba bean, the natural host from which the virus was first isolated. Sixteen lentil genotypes with different levels of susceptibility to bymv were selected to study the effect of mixed virus infection of bymv and faba bean necrotic yellows virus fbnyv, genus nanovirus, family nanoviridae under plastic house conditions. Present status of some virus diseases affecting legume.
Bean yellow mosaic virus bymv was first reported on french beans from the usa and the netherlands in 1925, and is now distributed worldwide. Faba bean necrotic yellows virus fbnyv was isolated from. The virus was detected by taselisa in faba bean muchamiel and peas only in. Nov 22, 2009 using monoclonal antibodies raised against a faba bean necrotic yellows virus fbnyv isolate from egypt and a faba bean necrotic stunt virus fbnsv isolate from ethiopia, a striking serological variability among nanovirus isolates from faba bean in morocco was revealed. Broad bean wilt virus taylor and stubbs, 1972 plantago ii virus uyemoto and provvidenti, 1974 patchouli mild mosaic virus natsuaki et al. Screening of lentil genotypes for resistance to bean.
Plants showing marked foliar mosaic symptoms, typical of viral. Blrv is another but is limited to fabacae faba bean, field pea, chickpea, and lentil, lucerne, clovers and summer legumes. Vegarocha s, gronenborn b, gronenborn am, camposolivas r. It has frequently been found infecting faba bean vicia faba l. A collection of 51 bean yellow mosaic virus bymv isolates was transmitted from infected trifolium subterraneum subterranean clover to lupinus angustifolius narrowleafed lupin by myzus persicae green peach aphid.
Faba bean necrotic yellows virus genus nanovirus requires a. Peas are reported to be infected with at least 30 viruses worldwide. Epidemiology and control of faba bean necrotic yellows virus. There are a large number of viruses damaging faba bean crop, such as bean yellow mosaic virs bymv, pea enation mosaicvirus pemv, bean leaf roll virus blrv, faba bean necrotic yellows virus fbnyv, true broad bean mosaic virus tbbmv, broad bean mottle. Faba bean is partially plot to act as virus spreader rows. Fab test article about fab test by the free dictionary.
Biological properties of necrotic and nonnecrotic strains of. Faba bean necrotic yellows virus fbnyv, blrv, bymv, and broad bean mottle virus bbmv. The coat protein cp gene nucleotide sequence of seven cpcsv isolates collected. Screening of lentil genotypes for resistance to bean yellow. Two distinct nanovirus species infecting faba bean in morocco.
It has a wide host range which includes the temperate pulses and pasture legumes, tropical legumes and ornamental hosts. They have a very wide host range and are transmitted by aphids in a nonpersistent manner. Distribution and incidence of faba bean necrotic yellows virus in. Cucurbit chlorotic yellows virus p22 protein interacts. Broad bean wilt virus an overview sciencedirect topics. Effects of seed dressing pesticides on the spread of faba. Molecular characterization of faba bean necrotic yellows viruses in tunisia. Proteomes faba bean necrotic yellows virus isolate sv29288 fbnyv. Information on the distribution of faba bean necrotic yellows virus fbnyv in spain was gathered by sampling different legume crops faba bean muchamiel, forage faba bean, pea and chickpea in four regions murcia, andalucia, castilla y leon, and extremadura. Clinkprotein, which is encoded by the singlestranded dna nanovirus faba bean necrotic yellows virus fbnyv, p0 protein encoded by the poleroviruses beet western yellows virus bwyv, cucurbit aphidborne yellows virus cabyv, brassica yellows virus bryv, p72 encoded by rice blackstreaked.
Bean yellow mosaic virus an overview sciencedirect topics. Faba bean necrotic yellows virus how is faba bean necrotic. Asia and africa accounted for 72% of the area and 80% of the production of dry faba bean grains faostat, 2012. Acheta domesticus volvovirus, a novel singlestranded. Additionally, a turnip yellows virusassociated rna was identified. First report of pea necrotic yellow dwarf virus in the. There are currently eight species in this genus including the type species subterranean clover stunt virus. One orf had a low identity to circovirus nucleotide sequences ns. Eight genotypes were highly resistant, showing no disease symptoms disease index di0, 24 genotypes were resistant di0.
Faba bean necrotic yellows virus expression system. Acquisition, retention and transmission of faba bean necrotic yellows virus by two of its aphid vectors, aphis craccivora koch and acyrthosiphon pisum harris. No serological relationships of these viruses to lettuce necrotic yellows virus have been reported. Pea necrotic yellow dwarf virus pnydv is a nanovirus that was first detected in pea crops pisum sativum in saxonyanhalt, germany in 2009 grigoras et al. Solution structure of the nuclease domain from the master replication initiation protein from the nanovirus faba bean necrotic yellows virus and comparison with geminivirus and circovirus. Polyclonal antibodies to the bacterially expressed coat. Pdf first report of faba bean necrotic yellows virus fbnyv. Fab test synonyms, fab test pronunciation, fab test translation, english dictionary definition of fab test.
Alfalfa mosaic virus amv was first identified infecting lucerne in the usa, and is now distributed worldwide. Biological properties of necrotic and nonnecrotic strains. Other viruses such as bean leaf roll luteovirus blrv, cucumber mosaic cucumovirus. Ascochyta fabae in faba bean compared to other diseases alternaria and cerco. The virus was detected by taselisa in faba bean muchamieland peas only in. Two divergent isolates of turnip yellows virus tuyv were identified in pea and rapeseed. Moreover, both faba bean necrotic yellows virus fbnv isolates grouped together in same clade expect in case of dnan where azerbaijani isolate is placed with bangladeshi isolates of mdv and egyptian isolate with two chinese and one japanese isolates of mdv that might be the result of possible reassortment reported to be occur in dnan of mdv. A highly divergent south african geminivirus species. Resistance in faba bean viciafaba to bean yellow mosaic.
Solution structure of the nuclease domain from the master replication initiation protein from the nanovirus faba bean necrotic yellows virus and comparison with geminivirus and circovirus domains. Using monoclonal antibodies raised against a faba bean necrotic yellows virus fbnyv isolate from egypt and a faba bean necrotic stunt virus fbnsv isolate from ethiopia, a striking serological variability among nanovirus isolates from faba bean in morocco was revealed. Whereas the cp tree is unrooted, the rep tree was rooted using the translated master rep sequence of faba bean necrotic yellows virus fbnyv. Chlorosis in new shoots, leaf rolling, red ringspots on leaves and plant stunting cucumber mosaic virus cmv. Additionally, a turnip yellows virus associated rna was identified. Aranzazu moreno, alberto fereres, in advances in virus research, 2012. There are currently six species in this genus including the type species subterranean clover stunt virus. The unique properties of this volvovirus suggested that it belongs to a new virus family or genus. Faba bean necrotic yellow virus fbnyv was the major cause of disappearance of faba bean from middle egypt. Bean yellow mosaic virus infecting broad bean in the green. A single rep protein initiates replication of multiple genome. Interestingly, infectivity of cloned viral dnas has only been achieved for a single nanovirus to date, namely faba bean necrotic yellows virus fbnyv, an expanding pathogen of legume crops, rendering it especially important and amenable to reverse genetics. Two divergent isolates of turnip yellows virus from pea.
Host range of faba bean necrotic yellows virus and potential yield loss in infected faba bean. It has also caused considerable damage and yield loss to faba bean crops in some southern regions of nsw and sa in recent years, as has infections of subterranean clover stunt virus scsv in southern vic. The natural and experimental host ranges of faba bean necrotic yellows virus fbnyv were in. Molecular characterization of faba bean necrotic yellows viruses in. Makkouk and others published first report of faba bean necrotic yellows virus fbnyv infecting chickpea cicer. Nanovirus is a genus of viruses, in the family nanoviridae. Department of virology, dlo research institute for plant protection. Bean yellow mosaic virus bymvbean yellow mosaic virus bymv was first reported on french beans from the usa and the netherlands in 1925 and is now distributed worldwide. The nearly complete genome sequences of the virus isolates share 93. The low incidence of cpcdv infection in chickpea and lentil plants with symptoms might be explained by infection with other viruses, such as blrv, pea enation mosaic virus.
Most of these viruses cause losses to the crops they infect. Faba bean necrotic yellows virus fbnyv belongs to the nanoviruses, plant. The 15 faba bean accessions had different resistance to bymv and bbsv based on disease index. A total of 605 faba bean genotypes was evaluated for reaction to a syrian isolate sv20585 of bean yellow mosaic potyvirus bymv using artificial inoculation under plastic house conditions. To obtain a better understanding of this nanovirus variability in morocco, the entire genomes of two serologically. Inoculation of a fbnyvsusceptible faba bean line at different growth stages under field. Fbnyv faba bean necrotic yellows virus acronymfinder. Pdf identification of faba bean necrotic yellows virus from faba. Similar results of expression of recombinant histagged cp in the insoluble fraction have been reported for several plant viruses, pelargonium zonate spot virus 27, faba bean necrotic yellows. Two distinct nanovirus species infecting faba bean in. The effects of two seeddressing pesticides in reducing the spread of aphidtransmitted faba bean necrotic yellows virus fbnyv, genus nanovirus, family nanoviridae and barley yellow dwarf virus pav bydvpav, genus luteovirus, family luteoviridae wer investigated under field conditions at mornag research station, tunisia, for two growing seasons 201220 and 202014, using artificial. First record of faba bean necrotic yellows virus and beet western yellows virus infecting faba bean in tunisia. Broad bean wilt virus 1 bbwv1 and bbwv2 are the two most important virus species in the genus fabavirus. Present status of some virus diseases affecting legume crops.
The cellcycle link clink protein of a nanovirus faba bean necrotic yellows virus was investigated that. Fab test definition of fab test by the free dictionary. Molecular characterization of faba bean necrotic yellows. Department of plant protection, faculty of agriculture, university of jordan, amman, jordan. Subterranean clover stunt virus type species, banana bunchy top virus, milk vetch dwarf virus and faba bean necrotic yellows virus fbnyv. Among faba bean viruses, bean yellow mosaic virus bymv family potyviridae. May 29, 2007 interestingly, infectivity of cloned viral dnas has only been achieved for a single nanovirus to date, namely faba bean necrotic yellows virus fbnyv, an expanding pathogen of legume crops, rendering it especially important and amenable to reverse genetics. Faba bean necrotic yellows virus fbnyv genus nanovirus. The effects of two seeddressing pesticides in reducing the spread of aphidtransmitted faba bean necrotic yellows virus fbnyv, genus nanovirus, family nanoviridae and barley yellow dwarf viruspav bydvpav, genus luteovirus, family luteoviridae wer investigated under field conditions at mornag research station, tunisia, for two growing seasons 201220 and 202014, using artificial. The virus is spread by a number of aphid species nonpersistently as.
The 6 isolates from vicia faba vicia faba subject category. Infected bean plants remain very stunted and yellowish, figure 10. Incidence and transmission of faba bean necrotic yellows. In 2016, pnydv was detected countrywide in both germany and austria not only on pea but also on faba bean vicia faba, vetch v. There are a large number of viruses damaging faba bean crop, such as bean yellow mosaic virs bymv, pea enation mosaicvirus pemv, bean leaf roll virus blrv, faba bean necrotic yellows virus fbnyv, true broad bean mosaic virus tbbmv, broad bean mottle virus bbmv and broad bean stain virus bbsv 22,2,25.
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